宋明辉^1，， 李琼琼^2，， 张易^1，， 宋金霞^3，， 施春雷^1，*， 史贤明^1，

（  1、上海交通大学农业与生物学院，中美食品安全联合研究中心，上海 200240；       2、上海市食品药品检验所,上海 200240；       3、青岛大学附属医院，青岛 266003；  ）

摘要： 金黄色葡萄球菌形成菌膜通常能够增强菌株的抗生素耐药性，这对治疗金黄色葡萄球菌相关感染疾病带来极大困难。本研究评价了中国地区金黄色葡萄球菌10个不同流行克隆世系共84株菌的菌膜形成能力，并对其耐药性进行了分析。结果表明不同克隆世系的菌膜形成能力具有明显差异。耐药性分析结果显示84株金黄色葡萄球菌分离株对红霉素(72.6%)和阿奇霉素(72.6%)的耐药性最强。值得注意的是，有24株菌(29.8%)显示了甲氧西林耐药性，其主要分布于克隆世系CC239-agr I和CC5-agr II。此外，本研究调查了16个耐药相关基因在不同克隆世系中的分布，结果显示耐药相关基因分布与克隆世系并没有明显的相关性。综上所述，本研究结果统计分析表明具有菌膜形成能力的金黄色葡萄球菌比不能形成菌膜的菌株通常携带更多的耐药相关基因(p<0.01)。
关键词： 金黄色葡萄球菌；菌膜；克隆世系；agr分型；耐药性；耐药相关基因

SONG Minghui^1,， LI Qiongqiong^2,， ZHANG Yi^1,， SONG Jinxia^3,， SHI Chunlei^1,*，SHI Xianming^1,

（  1、MOST-USDA Joint Research Center for Food Safety, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240；       2、Shanghai Institute for Food and Drug Control, Shanghai 201203；       3、The Affiliated Hospital of Qiingdao University, Qingdao 266003；  ）

Abstract： Biofilm formation usually increases antimicrobial resistance capacity of Staphylococcus aureus, which poses considerable challenges to successful eradication of staphylococcal infections. In this study, a panel of 84 different S. aureus isolates belonging to 10 dominant clonal lineages in China were characterized according to their biofilm-forming capacity, and antibiotic resistance pattern. It was shown that isolates of different clonal lineages had obvious variations in biofilm-forming capacity. Resistance to individual antibacterial agents was most frequently observed to erythromycin (72.6%) and azithromycin (72.6%). Oxacillin-resistant was found in 25 (29.8%) isolates belonging to CC239-agr I and CC5-agr II. All tested isolates were further screened for the presence of 16 resistance genes by PCR, and no significant correlation was found between clonal lineage and the distribution of resistance genes. Overall, our results indicate that biofilm producers of S. aureus have a greater likelihood to carry more antibiotic resistance genes than non-biofilm producers (p<0.01). 
Keywords： Staphylococcus aureus; biofilm; clonal complex; agr type; antibiotic resistance; resistance genes.

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